WORKSHOP PENGGUNAAN SARI DAUN PACAR KUKU (Lawsonia inermis L) SEBAGAI PENGGANTI SAFRANIN PADA PEWARNAAN GRAM

Sa’adah Siregar; Vincentia Ade Rizky; Visensius Krisdianilo; Neti Eka Jayanti; Morischa Deyla Sitanggang

doi:10.55681/ejoin.v1i12.1968

Authors

Sa’adah Siregar Institut Kesehatan Medistra Lubuk Pakam
Vincentia Ade Rizky Institut Kesehatan Medistra Lubuk Pakam
Visensius Krisdianilo Institut Kesehatan Medistra Lubuk Pakam
Neti Eka Jayanti Institut Kesehatan Medistra Lubuk Pakam
Morischa Deyla Sitanggang Institut Kesehatan Medistra Lubuk Pakam

DOI:

https://doi.org/10.55681/ejoin.v1i12.1968

Keywords:

Leaf Nail Henna (Lawsonia inermis L), Safranin, Gram Staining

Abstract

Gram staining is one way to differentiate gram-positive bacteria and gram negative bacteria. Gram staining in the laboratory is done with using a solution of gentien violet, iodine, alcohol, and safranin. Leaf henna nail (Lawsonia inermis L) is a plant that produces the reddish yellow molecule is called Lawsone. Additionally leaves henna nails It also contains anthocyanins which produce a reddish orange color. This Community Service aims to inform the audience about the benefits of henna leaf extract (Lawsonia inermis L) is effectively used as a substitute for safranin in gram staining. The results of the workshop show that the audience can understand the benefits of henna nail leaf extract as a substitute for safranin in gram staining, but this is only an alternative.

Downloads

Download data is not yet available.

References

Armanzah, R. S., & Hedrawati, T. Y. (2016). Pengaruh Waktu Maserasi Zat Antosianin Sebagai Pewarna Alami dari Ubi Jalar Ungu (Ipomoea batatas L. Poir). Seminar Nasional Sains Dan Teknologi, November, 1–10

Djaeni, M., Ariani, N., Hidayat, R., & Utari, F. D. (2017). Ekstraksi Antosianin dari Kelopak Bunga Rosella (Hibiscus sabdariffa L.) Berbantu Ultrasonik: Tinjauan Aktivitas Antioksidan Ultrasonic Aided Anthocyanin Extraction of Hibiscus sabdariffa L. Flower Petal: Antioxidant Activity. Jurnal Aplikasi Teknologi Pangan, 6(3), 71.

Enaru B, Drețcanu G, Pop TD, Stǎnilǎ A, Diaconeasa Z. Anthocyanins: Factors Affecting Their Stability and Degradation. Antioxidants (Basel). 2021 Dec 9;10(12):1967. doi: 10.3390/antiox10121967. PMID: 34943070; PMCID: PMC8750456.

Jawetz, Melnick, A., 2013. Mikrobiologi Kedokteran, Jakarta: Penerbit Buku Kedokteran EGC.

Komala, O., Yulianita, & Fuji, R. S. (2019). Activity Of Antifungal Ethanol Extract 50% And Ethanol 96% Leaf Henna Nail (Lawsonia inermis L) Against Trichophyton mentagrophytes. Ekologia : Jurnal Ilmiah Ilmu Dasar Dan Lingkungan Hidup, 19(1), 12–19.

Pratiwi, D. ayu novia. (2014). Aktivitas Antibakteri Ekstrak Etanol Daun Pacar Kuku (Lawsonia inermis L.). Naskah Publikasi, 2(1), 10

Raja, W., Ovais, M., & Dubey, A. (2013). Phytochemical Screening and Antibacterial Activity of Lawsonia inermis Leaf Extract Central Laboratory Facility , Chhattisgarh Council of Science and Technology ,. 4(1), 33–36.

Tripathi N, Sapra A. Gram Staining. 2023 Aug 14. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan–. PMID: 32965827.